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KMID : 0360419820180020045
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Korean Journal of Pharmacology
1982 Volume.18 No. 2 p.45 ~ p.50
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A New Hot-Plate Method using Threshold Temperature; its Application on the study of the Interaction between Naloxone or Diazepam and Morphine A New Hot-Plate Method using Threshold Temperature; its Application on the study of the Interaction between Nalox
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Abstract
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Yeum et al. formulated a new hot-plate method using the threshold temperature, and there are some controversies on the effects of naloxone and diazepam on the antinociceptive action.
In this paper, the comparison of three methods registering analgesic activity and the application of the new hot-plate method formulated by Yeum et al. on the study of the influences of naloxone and diazepam on the analgesic effect of morphine were tried in male mice.
The results obtained were summarized as follows;
1) The least-square regression lines of the morphine analgesia plotted against log-dose showed the correlation coefficient of above 0.90, but the competitive antagonism produced by naloxone (0.1 mg/kg) against the analgesia was mere prominently demonstrated by the new hot-plate method than the other methods: original hot-plate method and electrical stimulation method.
2) In the experiment using the new hot-plate method, the log dose-response curve of morphine (y=7.30 x+49.80, 1=0.998) was shifted to the right by the pretreatment of naloxone (0.1 mg/kg), but was slightly shifted to the left by the pretreatment of diazepam (2.5 mg/kg).
This study suggests that for the analgesia experiment, the new hot-plate method is superior to the original hot-plate method or the electrical stimulation method, and that the potentiative effect of diazepam on the morphine analgesia is not significant.
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